We investigated shoot production by Acacia tortilis under three distinct land-use types and browsing regimes in southeastern Botswana: a large mammal exclosure (UB Nature Reserve, UBNR), a conservation area (Gaborone Game Reserve, GGR), and a livestock area (Tlokweng Rangelands, TR). We applied one-way ANOVA to determine variation in shoot production within and amongst land-use types, and also across vertical browsing levels ("Low" , "Medium" and "Upper"). Mean shoot length varied significantly amongst the three habitats, being highest in GGR, TR, and lowest in UBNR. Spinescence (spine number and mass) differed significantly under the three land-use types, being highest in GGR, TR, and UBNR, respectively. Within land-use types, shoot length differed significantly between the three browsing levels. Shoot length declined from the "Upper" to the "Low" browsing levels or zones. Leaf dry-mass differed significantly between the three browsing levels, decreasing from the highest to the lowest levels. The highest number of thorns produced was significantly higher on the " Upper " browsing zones. Thorn mass did not vary significantly across the browsing zones. We postulate that differential browsing pressure elicited the variable response in shoot production across the three land-use categories, and further discuss the implications for wildlife and rangeland management. Keywords: habitat, herbivory, rangeland, savanna woodland, spinescence, wildlife.