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Robust abundance estimates of wild animal populations are needed to inform management policies and are often obtained through mark–recapture (MR) studies. Visual methods are commonly used, which limits data collection to daylight hours and good weather conditions. Passive acoustic monitoring offers an alternative, particularly if acoustic cues are naturally produced and individually distinctive. Here we investigate the potential of using individually distinctive signature whistles in a MR framework and evaluate different components of study design.

The waterhole fool-moon game count survey was conducted during the hottest period of the year (October) for a duration of 72 hours (3 days and 3 nights), which ensured that wildlife in the park is solely dependent on artificial water points. The survey was conducted at all the 13 water points (11 artificial and 2 fountains), with each water point having a team of eight people, working in shifts. The counting commenced on 12 October 2019, a day before the full moon and finished on 15 October 2019, a day after the full moon.

As part of the Kavango-Zambezi Transfrontier Conservation Area (KAZA TFCA) the Mudumu Complexes of the east Zambezi Region of Namibia are important areas for wildlife connectivity and dispersal. The national parks of the east Zambezi Region, i.e. Mudumu and Nkasa Rupara, are too small to maintain viable populations of some wildlife species, which depend on being able to move across a network of landscapes, such as the conservancies and woodland areas to connect to protected areas in neighbouring countries such as Angola, Botswana and Zambia.