Twelve new species of Salticidae are described from southern Africa. Five of them from Zimbabwe - Dendryphantes sanguineus sp. n. (♂, ♀), Heliophanus tenuitas sp. n. (♂), Langona fusca sp. n. (♀), Langelurillus sibandai sp. n. (♀), Plexippus tsholoshto sp. n. (♀), four from Namibia - Cembalea hirsuta sp. n. (♂), Evarcha karas sp. n. (♂), Langelurillus namibicus sp. n. (♀), Langona sabulosa sp. n. (♂, ♀), and three species - Langona tortuosa sp. n. (♂, ♀), Plexippus lutescens sp. n. (♂), Pseudicius matabelensis sp. n. (♂, ♀) from both countries. For Cembalea triloris Haddad & Wesołowska, 2011, Langona pilosa Wesołowska, 2006 and Heliophanus megae Wesołowska, 2003 missing females are described for the first time. Heliophanus lesserti Wesołowska, 1986, Holcolaetis zuluensis Lawrence, 1973, Mogrus matisi (berLand and Millot, 1941) and Phlegra simplex Wesołowska and Russell-Smith, 2000 are newly recorded for Zimbabwe, Langelurillus minutus Wesołowska and Cumming, 2011 and Pseudicius solitarius Haddad and Wesołowska, 2011 for Namibia, Mihroheros termitophagus Wesołowska and Cumming, 1999, Langona bethae Wesołowska and Cumming, 2011 and Parajotus refulgens Wesołowska, 1999 for Botswana. Keywords: arachnology, taxonomy, Salticidae, new species, Afrotropical Region.