The growth and expansion of human populations and resource demands is driving large scale fragmentation and loss of wildlife habitat, isolating wildlife populations and pushing many species towards extinction at local to global scales.  Attempts to promote connectivity between wildlife managed areas at transboundary scales has been proposed as a solution to negative effects associated with population isolation. Such approaches commonly require the maintenance of wildlife populations throughout human-dominated landscapes subject to various degrees of effective protection. The aims of this study are to (1) assess the status of the large carnivore guild throughout ten wildlife managed areas comprising the Zambian component of Kavango-Zambezi Transfrontier Conservation Area between Kafue National Park and the Simalaha Wildlife Recovery Sanctuary on the Zambezi River; (2) model habitat suitability and connectivity in this landscape for Lion, Leopard and Spotted hyena; and (3) develop a site-specific map of human footprint pressure for the landscape and test if it can be used a proxy for determining the occurrence of these three species. And further, explore if there are thresholds in human footprint pressure beyond which species are likely extirpated from wildlife managed areas. Methods included library studies to determine historical status of the large carnivore guild and twenty-six common prey species, spoor tracking in conjunction with qualitative surveys and supplemental data analysis to ascertain species current distribution, remote sensing with ground-truthing to build landcover maps, Maximum Entropy and Current Flow models, and extensive use of Geographic Information Systems.