Arid regions are home to unique fauna, flora, and vulnerable human populations, and present a challenge for sustainable land-use management. We undertook an assessment and valuation of three key services, grazing, tourism and water supply in the arid Succulent Karoo biome in western South Africa - a globally recognised biodiversity hotspot. We were looking for ways and values that could be used to promote conservation in this region through the adoption of sustainable land-use practices which have human welfare benefits. Our study adopted a variety of methods in valuing these services in developing ranges of values for these services. At the biome level, total annual values ranged from $ 19–114 million for grazing, $ 2–$ 20 million for tourism, and $ 300–3120 million for water. These values are generally low compared with values derived for other biomes and regions and do not adequately reflect known dependence and the importance of ecosystem services to the residents of this biome. The ecosystems here provide small but critical benefits enabling communities to sustain themselves and small changes in service levels can have major welfare effects. Highlighting these sensitivities will require finding more appropriate ways to link ecological and social factors. Keywords: Conservation, human wellbeing, resource limited, scarcity of services, sustainable land-use.