There are large gaps in our understanding of parasite–host nutrient relationships. Our goal was to evaluate transdermal water loss, parasite–host mineral relationships, and heterotrophy in the holoparasitic genus Hydnora. We estimated in situ transdermal water loss in Hydnora and measured nutrient profiles and δ13C and δ15N signatures for Hydnora and hosts in southern Africa and Madagascar. For comparison we also measured δ13C and δ15N for aerial hemiparasites at the same sites. Transdermal water loss in Hydnora ranged from 0.14 ± 0.02 to 0.38 ± 0.04 mg cm−2 h−1 and was comparable to transpiration rates for water conservative xerophytes. Concentrations of P and K were higher in Hydnora relative to CAM hosts; other mineral concentrations were significantly lower in the parasite or were not different. δ13C signatures of holoparasites and hemiparasites relative to their hosts reflected host metabolism and differences in commitment to heterotrophic C gain. Holoparasite δ13C values were significantly enriched (by 0.55‰ ± 0.23) compared to host shoot and depleted compared to host root tissues (by −0.97‰ ± 0.12). Holoparasite δ13C values were not significantly different compared to the estimated whole host δ13C value. δ15N values for holoparasites and hemiparasites were significantly correlated with hosts. The water conservative nature of Hydnora spp. combined with parasite–host mineral nutrition profiles are suggestive of active processes of solute uptake. Stable isotope fractionation in host tissues dictated significant differences between parasite and host (shoot and root) δ13C signatures. The confirmation of complete heterotrophy and the lack of a confounding transpiration stream may make Hydnora a promising model organism for the examination of parasite solute uptake. Keywords: Euphorbia, Stable isotopes, Transdermal water loss, Parasitic plant, Solute uptake.