Karyotypes from a natural population of Madoqua kirki damarensis in Namibia indicate a chromosomal complement of 2n = 48 (FN = 50). Comparisons of G- and C-bands with two previously characterized cytotypes (defined as cytotype A and B; 2n = 46-47) of captive M. kirki from East Africa indicate that one tandem fusion and one pericentric inversion separate cytotype A from M. k. damarensis (cytotype D). Two heterochromatic additions/deletions, one pericentric inversion, and an autosome-to-X chromosome translocation separate cytotype B from D. These data suggest an advanced stage of divergence in populations of M. kirki in Africa. Keywords: Madoqua kirki, karyotypes, chromosomal rearrangements.