The relationship between multi-year (1989-2003), herbaceous biomass and 1-km(2) Advanced Very High Resolution Radiometer (AVHRR) Normalized Difference Vegetation Index (NDVI) data in Kruger National Park (KNP), South Africa is considered. The objectives were: (1) to analyse the underlying relationship between NDVI summed for the growth season (Sigma NDVI) and herbaceous biomass in field sites (n=533) through time and (2) to investigate the possibility of producing reliable herbaceous biomass maps for each growth season from the satellite Sigma NDVI observations. Keywords: Herbaceous biomass, NDVI, Kruger National Park, South Africa, AVHRR.