This paper deals with specimen of spiders collected during a Namib desert expedition. The material taken by various members of the expedition reached a total of thirty-nine identifiable species of Arachnida and Myriopoda. The division between the fauna of the sand-dunes and that found on the rocky flats behind or to the east of the dried up Kuiseb River is a very sharp and definite one. As can be seen from the list, all the species taken in the sand-dunes are different from those found on the flats, a region covered with boulders of decomposing granitic rock, the bed of the Kuiseb River forming a clear-cut boundary between them. Only one species was not found either under stones on the flats or in the sand-dunes, this being the scorpion, Uroplectes otjimbinguensis, which was taken only in the river bed and appears to be associated with Acacia trees under whose bark it lives. Keywords: Spiders, Arachnida, Myriopoda, New species, Namib Desert.