Standard karyotypic data are presented for the species of the southern African rodent genus Gerbillurus. Gerbillurus paeba and G. tytonis have a diploid number of 36 and 68 autosomal arms. The karyotypes are morphologically similar to each other. G. vallinus and G. setzeri have a diploid number equaling 60, but the karyotypes differ in chromosome morphology. The autosomal arm number is 80 for the former and 76 for the latter species. Speculations on phylogenetic relationships are presented.