Determining wildlife numbers and their distribution patterns are an important and relevant factor for increasing scientific knowledge, to provide credible information for managerial decisions to conserve and protect biodiversity. This research was aimed at studying the distribution of wildlife species within the Fish River Canyon using different Geographical Information System (GIS) approaches and methods by analysing distribution patterns, slope and aspect analysis on which the animals were counted in, and favourable vegetation type from which different wildlife species were counted. The paper was also concerned with estimating wildlife population numbers within the reserve by extrapolating the numbers of wildlife counted in the surveyed areas to areas no surveyed based on the different vegetation coverage and different slope areas.