We examined patterns of cpDNA RFLP variability using 21 restriction endonucleases in 21 populations of Androcymbium that represent 12 endemic species distributed in the winter rainfall areas of South Africa to explore the diversification of the genus in its area of maximum species diversity. Our results are supportive of a diversification landscape characterized by continued opportunistic short-range invasion, naturalization, and rapid speciation, in which the selective action of the different environments where Androcymbium species occur determined their colonization success and subsequent short-range geographic expansion. The historical presence of fire, the constraint imposed by the low concentration of nutrients throughout southwestern South Africa and the different reproductive capabilities of Androcymbium species have also likely stimulated species' diversification. Our divergence time estimates bolster the view that speciation of South African Androcymbium initiated in the late Eocene, intensified in the Oligocene and proceeded more sporadically during the Miocene. These chronological estimates also substantiate the previous hypothesis that most lineages of Androcymbium in South Africa are much more ancient than their North African relatives, whose diversification began in the late Miocene-early Pliocene. Keywords: Androcymbium, Colchicaceae, Cape Floristic Region, cpDNA RFLPs, endemics, diversification.