Revision of the genus Latrunculia du Bocage, 1869, has resulted in 27 of 36 species considered to be valid. Many species have been reassigned to genera in the poecilosclerid family Podospongiidae, in particular, and many have been transferred to other Latrunculiidae genera. Latrunculia apicalis var. biformis (Kirkpatrick 1908) and Latrunculia apicalis var. basilis (Kirkpatrick 1908) have been elevated to full and separate species status, as L. biformis Kirkpatrick and L. basilis Kirkpatrick. Two new species have been described, from New Caledonia (L. novaecaledoniae sp. nov.) and the northeastern Pacific (L. austini sp. nov.). This revision also formally recognizes the two morphological subgroups triverticillata and spinispiraefera hypothesized by Alvarez et al. (2002), as the new subgenera Latrunculia (Latrunculia) subgen. nov. and Latrunculia (Biannulata) subgen. nov.. Keywords: Porifera, Sponges, Latrunculiidae, Biannulata, Subgenus latrunculia, New species, Poecilosclerida, Latrunculia du bocage.