A phylogenetic analysis of the small genus Pentatrichia, containing three species endemic to South Africa and Namibia, was undertaken using nuclear (ITS and 3' ETS) and chloroplast (trnT-trnL) DNA sequence data. Generic circumscription was examined via the inclusion of appropriate outgroup taxa (Anisothrix and Athrixia). Keywords: Anisothrix, Athrixia, ETS, Gnaphalieae, ITS, Molecular systematics, Pentatrichia, Phylogeny, southern Africa, trnT-trnL spacer.